Alteration in the distribution of basic soluble rat liver proteins during azo dye carcinogenesis.
نویسندگان
چکیده
Antibodies against rat liver // proteins and against a similar charge class of azoproteins have been labeled with fluorescein isothiocyanate (FITC), and the labeled antisera have been used as histoserological stains on unfixed frozen sections of rat liver. Fixation of antibody was defined by fluorescence microscopy and was used to determine the intracellular distribution of the appropriate antigens. FITC-labeled anti-/; protein stained the cytoplasm of both normal and 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB)-treated rat livers uniformly but did not stain 3'MeDAB-induced rat
منابع مشابه
Alteration in the Distribution of Basic Soluble Rat Liver Proteins during Azo Dye Carcinogenesis1
Antibodies against rat liver // proteins and against a similar charge class of azoproteins have been labeled with fluorescein isothiocyanate (FITC), and the labeled antisera have been used as histoserological stains on unfixed frozen sections of rat liver. Fixation of antibody was defined by fluorescence microscopy and was used to determine the intracellular distribution of the appropriate anti...
متن کاملZonal Electrophoresis of the Soluble Proteins of Liver and Tumor in Azo Dye Carcinogenesis.
A procedure has been developed for the zonal electrophoretic resolution of the sol uble proteins of rat liver with the use of a modified Porath column of ethanolyzed cellu lose. The mild method, with 750 mg. on less of protein, has been applied to various studies. An initial degree of resolution reproducibly separates the soluble liver proteins into all their principal charge classes with high ...
متن کاملEarly studies on hepatocarcinogenesis induced by amino azo dyes in the rat revealed an increase in DNA and a decrease in RNA per gm tissue in precancerous liver and hepatoma (11, 12), although occasional inconsistencies
azo dyes in the rat revealed an increase in DNA and a decrease in RNA per gm tissue in precancerous liver and hepatoma (11, 12), although occasional inconsistencies were observed. Recent studies (9) in which RNA and DNA were isolated and purified have confirmed the de crease in total RNA relative to DNA. In these studies O'Sullivan (9) found no significant alteration in nucleotide base composit...
متن کاملAntigenic changes in nonhistone proteins during azo dye hepatocarcinogenesis.
We have investigated the appearance of specific nonhistone proteins during azo dye-induced hepatocarcinogenesis in the rat. Groups of animals fed azo dye-containing diet were sacrificed at approximately 3-week intervals, portions of their livers were examined histologically, and the remaining material was fractionated into chromatin and cytoplasmic fractions. Livers of the azo dye-fed animals e...
متن کاملDye Carcinogenesis*
With the use of 2.1 M sucrose solution, nuclei were isolated from normal, control, and azo preneoplastic livers, and from azo dye-inducedliver tumors of rats. Four consecu tive extractions of nuclear sap in isotonic saline-phosphate buffer solubiized 49 and 37 per cent of the nitrogen of liver and tumor nuclei, respectively. Velocity sedimentation analyses revealed that the soluble nuclear macr...
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عنوان ژورنال:
- Cancer research
دوره 31 12 شماره
صفحات -
تاریخ انتشار 1971